Journal: bioRxiv

Article Title: Application of D4 Fluorescent Probes for Quantitative and Spatial Analysis of Cholesterol in Cells

doi: 10.64898/2026.04.01.715848

Figure Lengend Snippet: Sucrose gradient and western blot were performed on MDA-MB-231 cells without ( A. ) or with ( B.) D4-GFP probe. Flotillin 1 was used as a raft marker and Transferrin Receptor antibody was used as a non-raft marker. An anti-GFP antibody was used to detect the probe. Green arrow is placed at the size of the D4-GFP probe.

Article Snippet: Aliquots of 50-175 μg of lysate were immunoprecipitated for 2h at 4 °C with 0.75-1.5 μg of antibody mouse anti-GFP (Novus Biologicals, #9F9.F9).

Techniques: Western Blot, Marker

Journal: bioRxiv

Article Title: Application of D4 Fluorescent Probes for Quantitative and Spatial Analysis of Cholesterol in Cells

doi: 10.64898/2026.04.01.715848

Figure Lengend Snippet: A & B. MDA-MB-231 cells were incubated with or without D4-GFP, lysed and subjected to immunoprecipitation using and anti-GFP antibody (or Control IP). IP and first flow-through fractions were loaded on Western blot along with the total lysate (N=3). A. Representation blots B. Quantifications

Article Snippet: Aliquots of 50-175 μg of lysate were immunoprecipitated for 2h at 4 °C with 0.75-1.5 μg of antibody mouse anti-GFP (Novus Biologicals, #9F9.F9).

Techniques: Incubation, Immunoprecipitation, Control, Western Blot